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How To Reliably Test For Gmos

How To Reliably Test For Gmos - GRUDEN, KRISTINA; Eede, Guy van den; Plan, Damien; Morisset, Dany; Milavec, Mojca; Zel, Jana - ISBN: 9781461413899
Prijs: € 45,45
Levertijd: 3 tot 5 werkdagen
Bindwijze: Boek, Paperback
Genre: Laboratoriumtechniek
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Beschrijving

Provides The Key Topics Involved In Genetically Modified Organisms (gmos) Testing And Is A Source Of Information For Laboratories. This Book Focuses On Qualitative And Quantitative Real-time Pcr Analysis Relevant To Areas Where Detection And Identification Rely On Nucleic Acid-based Methods. It Also Focuses On The Methods For Testing.

Details

Titel: How To Reliably Test For Gmos
Auteur: GRUDEN, KRISTINA; Eede, Guy van den; Plan, Damien; Morisset, Dany; Milavec, Mojca; Zel, Jana
Mediatype: Boek
Bindwijze: Paperback
Taal: Engels
Aantal pagina's: 110
Uitgever: Springer-verlag New York Inc.
NUR: Laboratoriumtechniek
Afmetingen: 235 x 155 x 8
Gewicht: 182 gr
ISBN/ISBN13: 9781461413899
Intern nummer: 18224881

Inhoudsopgave

How to Reliably Test for GMOs 1(1)
Introduction 1(4)
Genetically Modified Organisms
1(2)
Presence of GMOs on the World Market
3(1)
Information on GMOs and Their Detection on the Web
3(2)
Legislation on GMO Labeling and GMO Detection
5(12)
Overview of the Global Situation of GMO Labeling Legislation
6(3)
The European Union Legislation on GMO Labeling and Traceability
9(3)
EU Legislation on GMO Detection
12(5)
Organization of the Laboratory and Quality Management System
17(12)
Introduction
17(1)
Personnel
18(1)
Accommodation and Environmental Conditions
18(1)
Test Methods and Method Validation
19(1)
Measurement Traceability and Reference Materials
20(5)
Sampling
25(1)
Handling of Test Items
25(1)
Assuring Quality of Testing
26(3)
Methods
29(8)
Introduction
29(1)
DNA-Based and Immunological Methods
29(1)
Singleplex, Multiplex Methods
30(1)
Detection Procedure Using DNA-Based Methods
31(4)
GMOs with Stacked Genes
35(1)
Availability of Methods
35(2)
Verification of Methods
37(16)
Introduction
37(2)
Method Validation
39(1)
Aspects of Modular Approach in Method Verification
39(1)
Strategy for Selecting the Methods to Be Implemented
40(1)
Verification Procedure
41(1)
Verification Plan
42(2)
Experimental Verification
44(8)
Data Administration
52(1)
Homogenization and DNA Extraction
53(4)
Introduction
53(1)
Homogenization
54(1)
Risk of Cross-Contamination
55(1)
DNA Extraction
55(2)
DNA Quantification
57(1)
Real-Time PCR
57(18)
Introduction
57(2)
qPCR Equipment
59(1)
Chemistries
60(1)
qPCR Setup, Analysis, and Interpretation
60(13)
Reporting
73(2)
Measurement Uncertainty
75(7)
Introduction
75(1)
MU in GMO Detection
76(5)
Reporting of MU
81(1)
MU and Compliance of Results with Legislation
81(1)
New Challenges
82(15)
Introduction
82(1)
Multiplex qPCR Methods
83(1)
Alternative PCR-Based Technologies
84(1)
Ready-To-Use Multitarget Analytical System
84(1)
Microarrays
85(1)
Digital PCR
86(1)
Sequencing
86(1)
Bioinformatics Support
87(10)
References
90(7)
Index 97

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