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Molecular cloning, expression and purification of novel lipase enzyme

Molecular cloning, expression and purification of novel lipase enzyme - Arman, Kaifee - ISBN: 9783659970849
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Beschikbaarheid: Levertijd tussen de 5 en 15 werkdagen. Geen retour recht.
Bindwijze: Boek, Paperback (11-11-2016)
Genre: Biomedische technologie
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Beschrijving

Shewanella putrefaciens is a gram negative bacterium. S.putrefaciens is also a facultative anaerobe with the ability to reduce iron and manganese as the terminal electron acceptor in the electron transport chain in both solid and liquid media. S.putrefaciens is also recognized by its bright pink colour. They are resistant to changeable environmental conditions. Lipases are also found to be widespread among bacteria, based on homology searches in protein databases and bacterial genomes. Lipase genes have been cloned from fungi and bacteria and then have been searched enzyme activity. In this study, we preferred S. putrefaciens ATCC 8071 because its lipase enzyme was not characterized before. Lipase genes in Shewanella putrefaciens were transferred into E. coli with molecular cloning techniques. Also, lipase enzyme was purified and characterized.

Details

Titel: Molecular cloning, expression and purification of novel lipase enzyme
auteur: Arman, Kaifee
Mediatype: Boek
Bindwijze: Paperback
Taal: Engels
Aantal pagina's: 104
Uitgever: LAP Lambert Academic Publishing
Publicatiedatum: 2016-11-11
NUR: Biomedische technologie
Afmetingen: 220 x 150
ISBN/ISBN13: 9783659970849
Intern nummer: 37436717

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