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Methods And Protocols

Alcohol - Nagy, Laura E. (EDT) - ISBN: 9781617378379
Prijs: € 160,65
Levertijd: 12 tot 15 werkdagen
Bindwijze: Boek
Genre: Biomedische technologie
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Sections Present Clearly Defined Models Of Ethanol Exposure, Recent Advances In The Development Of Specific Methodologies To Mimic The Impact Of Ethanol Metabolism In Cultured Cells, And Methodologies To Investigate A Variety Of Cells And Tissues That Are Known To Be Disrupted By Ethanol, Amongst Other Topics.


Titel: Alcohol
auteur: Nagy, Laura E. (EDT)
Mediatype: Boek
Taal: Engels
Aantal pagina's: 420
Uitgever: Humana Press Inc.
Plaats van publicatie: 01
NUR: Biomedische technologie
Afmetingen: 235 x 155
Gewicht: 664 gr
ISBN/ISBN13: 9781617378379
Intern nummer: 17347197


Contents§Preface §Contributors §Part I. Animal Models of Acute and Chronic Ethanol Exposure§Acute models of ethanol exposure to mice§Timothy P. Plackett and Elizabeth J. Kovacs§A voluntary oral-feeding rat model for pathological alcohol liver injury§George L. Tipoe, Emily C. Liong, Tung-Ming Leung,and Amin A. Nanji§Intragastric ethanol infusion model in rodents§Hide Tsukamoto, Hasmik Mikrtchyan and Alla Dynnyk§A practical method of chronic ethanol administration in mice§Ruth A Coleman, Betty M. Young, Lucas E. Turner and Robert T. Cook§Part II. Animal models of ethanol exposure during development§Analysis of ethanol developmental toxicity in Zebrafish§Robert L. Tanguay and Mark J. Reimers§The avian embryo in fetal alcohol research§Susan M. Smith§Artificial rearing§Hector D. Dominguez and Jennifer D. Thomas§Intragastric intubation of alcohol during the perinatal period§Sandra J. Kelly and R. Charles Lawrence§Part III. Cell culture approaches to studying ethanol exposure§9. Human monocytes, macrophages and dendritic cells alcohol treatment methods§Gyongyi Szabo and Pranoti Mandrekar§10. Generation and use of primary rat cultures for studies of the effects of ethanol§Amanda Lindke, Barbara Tremper-Wells and Michael W. Miller§11. Development and properties of HepG2 cells which constitutively express CYP2E1§Defeng Wu and Arthur I. Cederbaum§12. Modeling the impact of alcohol on cortical development in a dish: Strategies from mapping neural stem cell fate§Rajesh C. Miranda, Daniel R. Santillano, Cynthia Camarillo, and Douglas Dohrman§Part IV. Regulation of Specific Organ Systems in Response to Ethanol§13. Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayer§R.K. Rao§14. Alcohol-induced oxidative stress in the liver: in vivo measurements§Gavin E. Arteel§15. Isolation of Kupffer cells from rats fed chronic ethanol§Megan R. McMullen, Michele T. Pritchard and Laura E. Nagy §16. Dendritic cells in chronic in vivo ethanol exposure models§Kevin L. Legge and Annette J. Schleuter§17. Formation and immunological properties of aldehyde-derived protein adducts following alcohol consumption§Geoffrey M. Thiel, Lynell W. Klassen and Dean J. Tuma§18. Assessment of natural killer (NK) and NKT cells in murine spleens and livers§Michael R. Shey and Zuhair K. Ballas§19. Polyclonal and antigen-specific responses of T cells and T cell subsets§Betty M. Young, Susan Wiechert, Ruth A. Coleman, Prajwal Gurung, and Robert T. Cook§20. B Cell studies and chronic ethanol mice§Shilpi Verma, Carla-Maria A. Alexander, Michael J. Carlson, Lorraine T. Tygrett and Thomas J. Waldschmidt§21. Histological analysis of bone§Urszula T. Iwaniec, Thomas J. Wronski and Russell T. Turner§22. Assessing effects of alcohol consumption on protein synthesis in striated muscles§Thomas C. Vary and Charles H. Lang§23. Methods to investigate the effects of chronic ethanol on adipocytes§Becky M. Sebastian, Li Kang, Xiaocong Chen, and Laura E. Nagy§Part V. Proteomic and Genomic Approaches to Study Responses to Ethanol §24. Proteomic approaches to identify and characterize alterations to the mitochondrial proteome in alcohol liver disease§Shannon M. Bailey, Kelly K. Andrina, Aimee Landar, and Victor M. Darley-Usmar§25. Alcoholic liver disease and the mitochondrial ribosome: methods of analysis§Alan Cahill and Peter Sykora§26. Microarray analysis of ethanol-induced changes in gene expression§Robnet T. Kerns and Michael Miles


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