From the reviews: "This book provides background information and protocols for identifying, isolating, characterizing, and manipulating germline stem cells. This book is written for basic science and clinical researchers as well as those interested in development and reproductive biology. Biomedical scientists and clinicians will also appreciate the wealth of new information. This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody s Review Service, October, 2008)

Contents§Preface ..§Contributors .. §Part I Identification and Regulation of Germline Stem Cells in Model Organisms §The Development of Germline Stem Cells in Drosophila§David A. Dansereau and Paul Lasko ......§Analysis of the C. elegans Germline Stem Cell Region§Sarah L. Crittenden and Judith Kimble .§Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell§Shree Ram Singh and Steven X. Hou .§Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells§Wei Liu and Steven X. Hou §Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (the milkweed bug Oncopeltus fasciatus)§David C. Dorn and August Dorn §High Resolution Light Microscopic Characterization of Spermatogonia§Hélio Chiarini-Garcia and Marvin L Meistrich ..§Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes§Jens Ehmcke and Stefan Schlatt...........................................................................§Epigenetic Control in Male Germ Cells§Durga Prasad Mishra and Paolo Sassone-Corsi .§GDNF Maintains Mouse Spermatogonial Stem Cells in vivo and in vitro§Hannu Sariola and Tiina Immonen .. §Part II In vitro Culture and Applications of Germline Stem Cells§Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts§Ina Dobrinski and Rahul Rathi §Spermatogonial Stem Cell Transplantation, Testicular Function and Restoration of Male Fertility in Mice§Derek J. McLean ...§12 Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture§F. Kent Hamra, Karen M. Chapman, Zhuoru Wu, and David L. Garbers chrically Complex Trait§Jason D. Heaney and Joseph H. Nadeau §Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries§Antonin Bukovsky, Satish K. Gupta, Irma Virant-Klun, Nirmala B. Upadhyaya, Pleas Copas, Stuart E. Van Meter, Marta Svetlikova, Maria E. Ayala, and Roberto Dominguez .. §Index ..

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