PART ONE

Basic Microbiology

Introduction to Microbiology

1. Ubiquity of Microorganisms

2. Pure Culture and Aseptic Technique

3. Introduction to the Compound Light Microscope

4. The Oil Immersion Lens

Introduction to Staining of Microorganisms

5. Simple Stains: Positive and Negative Stains

6. Differential and Other Special Stains

Introduction to Microbial Growth

7. Chemically Defined, Complex, Selective, and Differential Media

8. Quantification of Microorganisms

Introduction to the Environment and Microbial Growth

9. Aerobic and Anaerobic Growth

10. The Effect of Incubation Temperature on Generation Time

Introduction to Control of Microbial Growth

11. Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time

12. Control of Microbial Growth with Ultraviolet Light

13. Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth

14. Antibiotics

15. Antiseptics and Disinfectants

Introduction to Microbial Genetics

16. Selection of Bacterial Mutants Resistant to Antibiotics

17. Transformation: A Form of Genetic Recombination

18. Gene Regulation: Induction and Catabolite Repression

PART TWO

The Other Microbial World

Introduction to the Other Microbial World

l9. Microscopic Identification of Fungi

20. Parasitology: Protozoa and Helminths

21. Titering Prokaryotic Viruses

PART THREE

Microbes and Humans

Introduction to Medical Microbiology

22. Normal Skin Biota

23. Streptococci and Respiratory Microorganisms

24. Identification of Enteric Gram-Negative Rods

25. Clinical Unknown Identification

PART FOUR

Immunology

Introduction to Some Immunological Principles and Techniques

26. Differential White Blood Cell Stains

27. Lysozyme, an Enzyme Contributing to Innate Immunity

28. Enzyme-Linked Immunoabsorbent Assay (ELISA)

PART FIVE

Public Health

Introduction to Public Health

29. Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques

30. Microbial Examination of Food

31. Epidemiology

PART SIX

Applications of Biotechnology

Introduction to Biotechnology

32. Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR)

33. Electrophoresis

34. Identification of Bacteria Using the Ribosomal Database Project

35. Microarray

PART SEVEN

Individual Projects

Introduction to the Individual Projects

36. Luminescent Bacteria: Bacteria That Produce Light

APPENDICES

1. Culture Media and Reagent Formulas

2. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual

3. Dilution Practice Problems

4. Alternative Procedures

5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size

6. History and Working Principles of Light Microscopy